DETECTION WITH THE POLYMERASE CHAIN REACTION OF HUMAN PAPILLOMAVIRUS DNA IN CONDYLOMATA ACUMINATA TREATED WITH CO2LASER AND MICROWAVE
- 1 March 1995
- journal article
- Published by Wiley in International Journal of Dermatology
- Vol. 34 (3) , 209-211
- https://doi.org/10.1111/j.1365-4362.1995.tb01572.x
Abstract
The recurrence rates of condyloma acuminata are high. The reasons for the relatively high relapse rates with different treatments are unknown. Twelve specimens of condylomata acuminata of the vulva were excised from 12 patients and divided into three parts. One part was untreated, the second and the third parts were treated with CO2 laser and microwave, respectively. DNA was then extracted from tissue by proteolytic digestion and amplified by the polymerase chain reaction. Dot blots were performed with the use of radiolabeled consensus and human papilloma virus (HPV) type-specific probes. HPV DNA was amplified in 100% of untreated specimens (6-HPV 6; 6-HPV 11), and in 83.3% and 50% of specimens treated with CO2 laser and microwave, respectively. There was a significant difference in detection between untreated and microwave-treated specimens (chi 2 = 4.18, P < 0.05). Microwave damages HPV DNA more effectively than CO2 laser.Keywords
This publication has 8 references indexed in Scilit:
- Detection with the polymerase chain reaction of human papillomavirus DNA in condylomata acuminata treated in vitro with liquid nitrogen, trichloroacetic acid, and podophyllinJournal of the American Academy of Dermatology, 1992
- Treatment of male genital condylomatous lesions by carbon dioxide laser after failure of previous nonlaser methodsJournal of the American Academy of Dermatology, 1991
- Primary advanced and local recurrent head and neck tumors: effective management with interstitial thermal radiation therapy.Radiology, 1990
- Risk of papillomavirus infection in carbon dioxide laser treatment of genital lesionsJournal of the American Academy of Dermatology, 1990
- Infectious papillomavirus in the vapor of warts treated with carbon dioxide laser or electrocoagulation: Detection and protectionPublished by Elsevier ,1989
- Papillomavirus in the vapor of carbon dioxide laser-treated verrucaeJAMA, 1988
- Carbon dioxide laser irradiation of bacterial targets in vitroJournal of Hospital Infection, 1987
- The efficacy of the CO2 laser in the sterilization of skin seeded with bacteria: Survival at the skin surface and in the plume emissionsThe Laryngoscope, 1985