SPECTROSCOPIC AND ORIENTATIONAL PROPERTIES OF CHLOROPHYLLaAND CHLOROPHYLLbIN LIPID MEMBRANES

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Abstract
Linear dichroism and fluorescence depolarization measurements on chlorophyllaand chlorophyllbmolecules incorporated into macroscopically oriented multibilayers of the plant lipid digalactosyldiacylglycerol (DGDG) are presented. The results are combined with measurements of fluorescence anisotropy to obtain both the order parameters describing the orientational statistics and the directions of the absorption and emission transition moments in the frame of the molecules. The problem presented by the overlapping nature of the absorption and emission bands is overcome by determining the fluorescence depolarization at the two maxima of the emission spectrum.