IDENTIFICATION OF STREPTOCOCCI IN BACTERIAL MIXTURES AND CLINICAL SPECIMENS WITH FLUORESCENT ANTIBODY

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Abstract
Results of the application of the Coons fluorescent antibody technique to the rapid diagnosis of acute streptococcal pharyngitis are presented. Smears prepared with group A streptococci of types 2, 4, 12, 18, 33, and 39, and the Red Lake strain, were specifically stained with fluorescein labeled group A antisera. Two strains of group C streptococci stained faintly whereas strains of group D and G did not stain. Other bacterial species found in throat smears gave no fluorescein staining. Direct throat smears were obtained from 49 patients with acute pharyngitis from whom beta-hemolytic streptococci were isolated; 23 were group A, 10 were group C, and the remaining 16 were neither of these groups. Fluorescein stained Streptococcal chains were observed in the throat smears of 30 out of the 33 patients infected with groups A and C streptococci. Such smears from 50 patients with pharyngitis from whom no beta-hemolytic streptococci could be isolated were negative by fluorescein-antibody staining.

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