Direct Incorporation of Glucosamine and N -Acetylglucosamine into Exopolymers by Gluconacetobacter xylinus (= Acetobacter xylinum ) ATCC 10245: Production of Chitosan-Cellulose and Chitin-Cellulose Exopolymers

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Abstract
Gluconacetobacter xylinus (=Acetobacter xylinum) ATCC 10245 incorporated 2-amino-2-deoxy-d-glucose (glucosamine) and 2-acetamido-2-deoxy-d-glucose (N-acetylglucosamine), but not 3-O-methyl-d-glucose or 2-deoxy-d-glucose into exopolymers. Incorporation was confirmed by gas chromatography with and without mass spectrometry, Fourier transform infrared, and 1H nuclear magnetic resonance. The average molar percentage of glucosamine andN-acetylglucosamine in the exopolymers was about 18%.