Assays of three carcinogen/non‐carcinogen chemical pairs for in vivo induction of chromosome aberrations, sister chromatid exchanges and micronuclei

1 January 1989

journal article
research article
Published by Wiley in Environmental and Molecular Mutagenesis

Vol. 14 (4) , 207-220
https://doi.org/10.1002/em.2850140402

Abstract

Three pairs of structurally similar carcinogenic/ non‐carcinogenic chemicals were tested for in vivo genotoxic activity in B6C3F1 mice. The carcinogenic/non‐carcinogenic pairs, respectively, were o‐toluidine hydrochloride/o‐anthranilic acid, 4‐chloro‐o‐phenylenediamine/4‐nitro‐o‐phenylenediamine, and 3‐(chloromethyl)pyridine hydrochloride/2‐(chloromethyl)pyridine hydrochloride. Bone marrow cells from mice given intraperitoneal injections of up to the maximum tolerated dose were evaluated for chromosomal aberration, sister chromatid exchange, and micronucleus induction, o‐anthranilic acid and o‐toluidine hydrochloride did not increase the frequency of chromosomal aberrations or micronuclei. o‐Toluidine hydrochloride increased the frequency of sister chromatid exchanges in two successive trials, while o‐anthranilic acid had a positive effect on sister chromatid exchanges in two of three trials. Both 2‐(chloromethyl) and 3‐(chloromethyl)pyridine hydrochloride were negative for all three endpoints. Assays for chromosomal aberrations and micronuclei each distinguished between 4‐chloro‐o‐phenylenedi‐amine and its non‐carcinogenic companion, 4‐nitro‐o‐phenylenediamine. In the aberration test, 4‐chloro‐o‐phenylenediamine produced a few cells with very large numbers of aberrations rather than an even distribution of damage among cells.

Keywords

This publication has 34 references indexed in Scilit:

Tables for determining the statistical significance of mutation frequencies
Published by Elsevier ,2003
Genotoxic potency of three quinoline compounds evaluated in vivo in mouse marrow cells
Environmental and Molecular Mutagenesis, 1989
‘A Failed Paradigm: Carcinogenesis is More Than Mutagenesis’
Mutagenesis, 1988
Improved sister-chromatid differentiation using paraffin-coated bromodeoxyuridine tablets in mice
Mutation Research Letters, 1983
The induction of bacterial mutation and hepatocyte unscheduled DNA synthesis by monosubstituted anilines
Environmental Mutagenesis, 1983
Chromosomal analysis in vinyl chloride exposed workers: Comparison of the standard technique with the sister-chromatid exchange technique
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1981
Micronucleus test and bone-marrow chromosome analysis
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1981
Diethylstilbestrol‐diphosphate induces chromosomal aberrations but not sister chromatid exchanges in murine bone marrow cells in vivo
Environmental Mutagenesis, 1981
Dose response at low doses of X-irradiation and MMS on the induction of micronuclei in mouse erythroblasts
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1976
A correlative study on the genetic damage induced by chemical mutagens in bone marrow and spermatogonia of mice
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1976