The immunoassay of gibberellins

Abstract
Immunological assays for the detection of picogram amounts of gibberellins A1, A3, A4, A7, A9 and A20 are described. Antisera of high affinity (Ka over 1010 l mol-1) were raised in rabbits by immunization against carboxyl-coupled gibberellin-bovine-serum-albumin conjugates and used in combination with tritiated gibberellins or gibberellin derivatives of high specific radioactivity (over 1014 Bq mol-1). The assays allow gibberellin quantitation in as little as 1 mg of vegetative plant tissue.