Enzymatic thiolation of E. coli sRNA.
- 1 May 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 55 (5) , 1169-1174
- https://doi.org/10.1073/pnas.55.5.1169
Abstract
In whole soluble ribonucleic-acid(sRNA), the pyrimidine S acceptor which yields 4-thiouridylic acid is uracil and not cytosine. Incubation of a dialyzed cell-free extract from Escherichia coli with sRNA and S35-cystine leads to labeling of the thionucleotides of the sRNA. The enzymatic reaction requires adenosine triphosphate (ATP), MgCl2, and pyridoxal phosphate. The E. coli sRNA serves as a good S acceptor substrate, while E. coli ribosomal RNA, periodate-treated E. coli sRNA, yeast or rabbit liver sRNA, and polyuridylic acid are inactive.Keywords
This publication has 11 references indexed in Scilit:
- ACETYLORNITHINASE OF ESCHERICHIA COLI: PARTIAL PURIFICATION AND SOME PROPERTIESPublished by Elsevier ,2021
- RNA thiolase: the enzymatic transfer of sulfur from cysteine to sRNA in Escherichia coli extracts.Proceedings of the National Academy of Sciences, 1966
- The origin of the sulfur in s-RNABiochemical and Biophysical Research Communications, 1965
- The behavior of 4-thiouridine in the E.coli s-RNA moleculeBiochemical and Biophysical Research Communications, 1965
- Nucleotide Sequences in the Yeast Alanine Transfer Ribonucleic AcidJournal of Biological Chemistry, 1965
- A reversible oxidative in activation of specific transfer RNA species.Proceedings of the National Academy of Sciences, 1965
- Studies on soluble ribonucleic acid of rabbit liver III. Preparation and properties of rabbit-liver soluble RNABiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1962
- A NEW ENZYME OF RNA SYNTHESIS: RNA METHYLASEProceedings of the National Academy of Sciences, 1962
- Pseudouridine, a Carbon-Carbon Linked Ribonucleoside in Ribonucleic Acids: Isolation, Structure, and Chemical CharacteristicsJournal of Biological Chemistry, 1960
- The structure of ribonucleic acids. 1. Cyclic nucleotides produced by ribonuclease and by alkaline hydrolysisBiochemical Journal, 1952