Identification and Quantification of Mould Spores by Scanning Electron Microscopy (Sem): Analysis of Filter Samples Collected in Norwegian Saw Mills

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Abstract
The use of scanning electron microscopy (SEM) for identification and quantification of mould spores collected on filters in the sorting and trimming plants of Norwegian saw mills is described. Moulds were cultured and identified by their macro- and microscopical features. The moulds present consisted mainly of Aspergillus fumigatus, Paecilomyces variotii and Rhizopus rhizopodiformis. Spore samples of the identified moulds were prepared and investigated by SEM. The morphological differences between spore types were sufficient to allow identification of 95% of the spores collected in one mill. The relative precision of the determination of the spore concentration by SEM was 14% when 250 spores were counted.

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