Biosynthesis of the Enediyne Antitumor Antibiotic C-1027
Top Cited Papers
- 16 August 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 297 (5584) , 1170-1173
- https://doi.org/10.1126/science.1072110
Abstract
C-1027 is a potent antitumor agent with a previously undescribed molecular architecture and mode of action. Cloning and characterization of the 85-kilobase C-1027 biosynthesis gene cluster from Streptomyces globisporus revealed (i) an iterative type I polyketide synthase that is distinct from any bacterial polyketide synthases known to date, (ii) a general polyketide pathway for the biosynthesis of both the 9- and 10-membered enediyne antibiotics, and (iii) a convergent biosynthetic strategy for the C-1027 chromophore from four building blocks. Manipulation of genes governing C-1027 biosynthesis allowed us to produce an enediyne compound in a predicted manner.Keywords
This publication has 24 references indexed in Scilit:
- The Calicheamicin Gene Cluster and Its Iterative Type I Enediyne PKSScience, 2002
- Formation of β-Hydroxy Histidine in the Biosynthesis of Nikkomycin AntibioticsChemistry & Biology, 2002
- Production of Polyunsaturated Fatty Acids by Polyketide Synthases in Both Prokaryotes and EukaryotesScience, 2001
- The specificity-conferring code of adenylation domains in nonribosomal peptide synthetasesChemistry & Biology, 1999
- Modulation of Polyketide Synthase Activity by Accessory Proteins During Lovastatin BiosynthesisScience, 1999
- DNA-damaging Enediyne CompoundsPublished by Elsevier ,1999
- Biosynthesis of esperamicin A1, an enediyne antitumor antibioticJournal of the American Chemical Society, 1993
- Biosynthesis of dynemicin A, a 3-ene-1,5-diyne antitumor antibioticJournal of the American Chemical Society, 1992
- Chemistry and Biology of the Enediyne Anticancer AntibioticsAngewandte Chemie International Edition in English, 1991
- Biosynthesis of NCS Chrom A, the chromophore of the antitumor antibiotic neocarzinostatinJournal of the American Chemical Society, 1989