Continuous Particle Electrophoresis: A New Analytical and Preparative Capability

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Abstract
New differential migration techniques are breaching a barrier in terms of particle size in mixtures to be fractionated. Thus electrophoresis in free media is being applied to a wide variety of particles, mainly of biological interest, ranging from viruses to whole tissue cells and from 100 A to tens of microns in dimensions. A new technique of high-speed continuous electrophoresis is discussed which appears to offer a new order of capability for fractionating and studying of particles. An analysis is presented of certain considerations underlying this capability, in terms of instrument-response time, resolution, and sample-handling rate. Band broadening due to diffusion and velocity-profile effects is discussed in some detail and related to performance. Factors involved in making absolute mobility measurements are considered.