Delineation of a cross-reactive idiotype on human autoantibodies with antibody against a synthetic peptide.

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Abstract
Antibody against a cross-reactive idiotype (CRI) on human IgM-rheumatoid factor (RF) antibodies was induced by immunization of rabbits with a synthetic peptide (PSL2) corresponding to the 2nd complementarity-determining region (CDR), and adjacent amino acid residues of the .kappa. L chain of the IgM-Rf Sie. The anti-peptide antibody bound efficiently to IgM-RF proteins known to share a cross-reactive idiotype, and to their isolated .kappa. chains. The anti-CRI was absorbed by, and eluted from, a peptide-Sepharose affinity column. The antibody activity was inhibited by the free peptide in solution. The antipeptide antibody identifies a public idiotype on human IgM-RF, that is largely dependent on the primary sequence of the 2nd CDR of the L chain. Such peptide-induced antiidiotypes of predefined specificity may facilitate studies of the molecular basis of idiotypic cross-reactions, the inheritance and somatic diversification of antibody molecules and the regulation of the idiotypic network.

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