Inhibition of monocyte and polymorphonuclear granulocyte immune phagocytosis by monoclonal antibodies specific for FcγRI, II and III
- 22 January 1997
- journal article
- research article
- Published by Springer Nature in Annals of Hematology
- Vol. 74 (1) , 15-22
- https://doi.org/10.1007/s002770050249
Abstract
We tested two Fcγ receptor I (FcγRI); six FcγRII; and six FcγRIII-specific monoclonal antibodies (mAb) for their capacitiy to inhibit monocyte and polymorphonuclear granulocyte (PMN) immune phagocytosis which is mediated by FcγR. We used human red blood cells (rbc) coated with hIgG1 or mIgG1 as FcγRI- and FcγRII-specific target cells, respectively. The FcγRI-specific mAbs 22.2 and 32.2 did not inhibit FcγRI- or FcγRII-specific monocyte immune phagocytosis. The FcγRII-specific mAbs IV.3, CIKM5, FLI8.2, FLI 8.26, 2E1, and 41H16 inhibited FcγRII-specific monocyte immune phagocytosis in all FcγRIIa high-responder (HR) individuals but did not inhibit FcγRI-specific phagocytosis. Using PMN, FLI8.2 and 2E1 only partially inhibited phagocytosis in HR individuals, but the FcγRIII-specific mAbs 3G8, DJ130c, MFM-154, B88-9 and MG38 completely inhibited FcγRII-specific phagocytosis if the corresponding antigen was available on the cell surface. In these cases phagocytosis inhibition may be explained by cross-linking of FcγRII and FcγRIII via one antibody molecule, with the Fab portion binding to FcγRIII and the Fc portion binding to FcγRII.Keywords
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