Minimal Vitamin Requirements of Rabbit Fibroblasts, Strain RM3-73

Abstract
A strain of rabbit fibroblasts designated RM3-73 required folic acid, nicotinamide, pantothenic acid, pyridoxal, riboflavin, and thiamin for continuous proliferation in a medium containing 2% (v/v) dialyzed serum as the only undefined constituent. The concentration of vitamins which permits maximal proliferation ranged from 10-3 to 10-4 millimoles per liter as determined by propagating the cells serially in media containing graded concentrations of each compound.