QUANTITATIVE ISOLATION AND ASSAY OF NEUTRAL GLYCERIDE GLYCEROL AND PHOSPHOGLYCERIDE GLYCEROL

Abstract
Neutral glyceride glycerol and phosphoglyceride glycerol obtained by acid-catalyzed methanolysis of 10-100 mg quantities of mixed lipids are separated by paper chromatography. Quantitative elution of the compounds is followed by a periodate oxidation – chromotropic acid assay of the released formaldehyde. Application of this method to the assay of carbon-14-labelled material permits accurate specific activity determinations by radioactivity measurements on phosphoglycerol and on glycerol complexed with sodium borate.