Production and characterization of a monoclonal antibody to bovine β-crystallin

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Abstract
A monoclonal antibody to a bovine lens 27K .beta.-crystallin polypeptide was produced from a rat .times. mouse hybridoma. The antibody reacts with the 27K polypeptide of bovine, mouse, rat and human lenses, but does not react to the 27K polypeptide of monkey lens nor does it reach with any component in the Philly mouse lens which is missing the 27K polypeptide. The antibody does not recognize any of the other major bovine .beta.-crystallin polypeptides but does recognize a large number of native .beta.-crystallin proteins. The antigenic specificity and species cross-reactivity of this antibody provides an excellent opportunity to study many aspects of lens development and cataractogenesis.