Purification and Properties of Human Deoxycytidine Kinase

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Abstract
In tissues with active deoxyribonucleoside salvage pathways, such as the thymus and the spleen, it is likely that the salvage of extracellular deoxyribonucleosides is a major source of precursors for DNA — synthesis. Direct reduction of ribonucleotides to the corresponding deoxyribonucleotides via ribonucleotide reductase is the only other possible route for the synthesis of deoxynucleosidetriphosphates. There is evidence that in S-phase thymocytes, ribonucleotide reduction is restricted mainly to the synthesis of purine dNTPs, while pyrimidine dNTPs are synthesized primarily by extracellular deoxyribonucleoside salvage (1). We report, here, the purification procedure and some properties of pure human deoxycytidine kinase, one of the principal enzyme responsible for deoxyribonucleoside phosphorylation in human tissues (2-4).

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