The inactivation of ribonuclease during the isolation of ribonucleic acids and ribonucleoproteins from yeast

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Abstract
Nucleic acid-hydrolysing enzymes, which are present in yeast extracts, were activated by increasing ionic strength. They were inhibited by 0.6 mM-Zn2+ ions at I 0.1 but were not completely inhibited by this and higher concentrations of Zn2+ ions at higher ionic strengths. Most of the activity was probably due to a ribonuclease. The ribonuclease and also proteolytic enzymes were removed from yeast extracts by adsorption on bentonite. The consecutive use of Zn2+ ions and bentonite provided a mild method for the isolation of ribonucleic acids and ribonucleoprotein in which the ribonucleic acids had not been enzymically degraded.