Digitonin‐precipitable sterols as a measure of cholesterol biosynthesis: Contradictory results
- 1 September 1983
- Vol. 18 (9) , 641-649
- https://doi.org/10.1007/bf02534676
Abstract
In experiments with 4 different types of cells, we evaluated the cholesterogenic activity by incorporation of14C-acetic acid into cholesterol and digitonin-precipitable sterols. In every case, the cholesterogenesis appeared considerably faster when expressed as digitonid than when expressed as real cholesterol production, and sometimes the data obtained by the 2 methods were contradictory. Detailed analysis of both digitonid components and nonprecipitable radioactive metabolites showed that a very variable fraction of methyl sterols (including bifunctional methyl sterols) co-precipitates with the C-27 sterols. In cholesterol regulation studies and particularly when the cells exhibit a low cholesterogenesis, the digitonin method is unsuitable and can lead to erroneous interpretations.This publication has 13 references indexed in Scilit:
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