High-Throughput One-Bead-One-Compound Approach to Peptide-Encoded Combinatorial Libraries: MALDI-MS Analysis of Single TentaGel Beads
- 18 January 2003
- journal article
- Published by American Chemical Society (ACS) in Journal of Combinatorial Chemistry
- Vol. 5 (2) , 125-137
- https://doi.org/10.1021/cc020083a
Abstract
The identification of pharmacologically promising compounds (lead compounds) from combinatorial libraries is frequently limited by the throughput of the analytical technique employed. Fourier transform mass spectrometry (FTMS) offers high sensitivity, mass accuracy (m/Δm > 500 000), and sequencing capabilities. A rapid and efficient method for high-throughput analysis of single beads from peptide-encoded combinatorial libraries with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is presented. Encoding peptides on single beads are identified and structurally characterized by MALDI time-of-flight (TOF) and ultrahigh-resolution MALDI Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. A strategy of on-probe sample preparation is developed to minimize handling of the beads.Keywords
This publication has 27 references indexed in Scilit:
- Mass spectrometry and combinatorial chemistry: a short outlineJournal of Mass Spectrometry, 2001
- Combinatorial Chemistry and Mass Spectrometry in the 21st Century Drug Discovery LaboratoryChemical Reviews, 2001
- Exploring the limit of detection in biomolecular interaction analysis mass spectrometry (BIA/MS): detection of attomole amounts of native proteins present in complex biological mixturesAnalytica Chimica Acta, 2000
- Mass spectrometry in combinatorial chemistryMass Spectrometry Reviews, 2000
- Minimizing Peak Coalescence: High-Resolution Separation of Isotope Peaks in Partially Deamidated Peptides by Matrix-Assisted Laser Desorption/Ionization Fourier Transform Ion Cyclotron Resonance Mass SpectrometryAnalytical Chemistry, 1997
- An expanded nomenclature scheme for labeling peptide fragmentations and its use with ‘AMASS’, a computer program for generating all possible fragment ion structures from known precursorsJournal of Mass Spectrometry, 1993
- Generation and use of synthetic peptide combinatorial libraries for basic research and drug discoveryNature, 1991
- Analysis of the amino acid sequence of peptides by mass spectrometry An ion notation proposalInternational Journal of Peptide and Protein Research, 1990
- Solid phase peptide synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acidsInternational Journal of Peptide and Protein Research, 1990
- Evaluation of fast atom bombardment mass spectrometry for sequence determination of peptidesJournal of Mass Spectrometry, 1985