HETEROAGGLUTINATION OF DISSOCIATED SPONGE CELLS

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Abstract
Results of experiments carried out on a variety of sponges collected round Agar''s Is. showed that separated cells displayed amoeboid movements and coalesced with each other to form aggregates. The process of reunion was essentially similar in all sponges tested but there was considerable difference in the velocity and duration of the amoeboid movements and in size and number of aggregates. Changing the pn of the water and increasing its Ca content did not prevent the detachment of aggregates from the substratum within 24 hrs. Suspensions of Reniera and Pachychalina showed that the presence of foreign cells inhibited the amoeboid movements of the archaeocytes and prevented their aggregation. The degree of reciprocity varied, but in all cases mixed suspensions caused immediate cytolysis accompanied by agglutination of suspended material. Heteroagglutination is not influenced by pn values of suspensions but brought about by some substance of unknown nature which causes cytolysis. Results of experiments are tabulated.

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