Genetic instability of the Streptomyces chromosome

Open Access

1 February 1998

journal article
review article
Published by Wiley in Molecular Microbiology

Vol. 27 (2) , 239-246
https://doi.org/10.1046/j.1365-2958.1998.00652.x

Abstract

The Streptomyces wild‐type chromosome is linear in all examples studied. The ends of the chromosome or telomeres consist of terminal inverted repeats of various sizes with proteins covalently bound to their 5′ ends. The chromosome is very unstable and undergoes very large deletions spontaneously at rates higher than 0.1% of spores. Frequently, the telomeres are included in the deletions. Loss of both telomeres leads to circularization of the chromosome. The wild‐type chromosome can also be circularized artificially by targeted recombination. Spontaneously or artificially circularized chromosomes are even more unstable than the linear ones. High‐copy‐number tandem amplifications of specific chromosomal regions are frequently associated with the deletions. RecA seems to be involved in the amplification mechanism and control of genetic instability.

Keywords

This publication has 48 references indexed in Scilit:

Mutational analysis of the Streptomyces lividans recA gene suggests that only mutants with residual activity remain viable
Molecular Genetics and Genomics, 1997
Dynamic bacterial genome organization
Molecular Microbiology, 1997
Nucleotide sequence analysis of the junctions of the terminal inverted repeats of the Streptomyces lividans linear chromosome
Molecular Genetics and Genomics, 1997
Artificial circularization of the chromosome with concomitant deletion of its terminal inverted repeats enhances genetic instability and genome rearrangement in Streptomyces lividans
Molecular Genetics and Genomics, 1997
Chromosomal deletions in Streptomyces griseus that remove the afsA locus
Molecular Genetics and Genomics, 1997
Isolation and characterization of a mutator strain of Streptomyces ambofaciens ATCC23877 exhibiting an increased level of genetic instability
Canadian Journal of Microbiology, 1996
The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA
Molecular Microbiology, 1996
The use of an improved transposon mutagenesis system for DNA sequencing leads to the characterization of a new insertion sequence of Streptomyces lividans 66
Gene, 1996
Primary structure analysis of a duplicated region in the amplifiableAUD6locus ofStreptomyces ambofaciensDSM40697
FEMS Microbiology Letters, 1993
Ultraviolet light, mitomycin C and nitrous acid induce genetic instability in Streptomyces ambofaciens ATCC23877
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1993