Canine Parvovirus: a Biochemical and Ultrastructural Characterization

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Abstract
A canine virus derived from a diseased dog was plaque-purified and characterized in detail. Analysis of infected [SV40-transformed dog kidney] cells demonstrated that virus antigen accumulated in the nucleus at 12-24 h post-infection and the cytopathology at the ultrastructural level was diagnostic of a parvovirus infection. The purified virus particles were 23-26 nm in diameter and banded at a density of 1.44 g/ml in CsCl. Detailed biochemical analysis revealed a single-stranded DNA genome and 3 structural protiens of MW 82,300, 67,300 and 63,500. All of the data presented are consistent with the classification of this virus as a parovirus.