PREPARATION AND EFFICACY OF ANTIHERPES TYPE-1 AND TYPE-2 SUBUNIT VACCINES

  • 1 September 1986
    • journal article
    • research article
    • Vol. 30  (5) , 402-410
Abstract
DNA-free antiherpes subunit vaccines were prepared from diploid human embryonic lung cells infected either with type 1 or type 2 herpes simplex viruses (HSV). Virion and membrane-bound virus-specific glycoproteins were solubilized with Nonidet P-40 and separated by ultracentrifugation. The antigenic properties of the vaccine were tested in guinea pigs. Antibody response was followed by virus neutralization and complement fixation. The vaccine itself was low-immunogenic, however its immunogenicity has considerably increased by usage of suitable adjuvants. In virus neutralization test higher antibody titer was found against homologous virus. The antibody response was related to protein content and to the frequency of vaccination.