Topology of RbsC, the Membrane Component of the Escherichia coli Ribose Transporter

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Abstract
The topology of RbsC, the membrane component of the ribose transporter in Escherichia coli , has been determined by using 34 single-cysteine mutants and a modified fluorescence labeling technique designated multiplex labeling. This technique gives topology, expression, and localization information for a membrane protein from a single batch of bacterial cells. The results indicate that RbsC contains 10 transmembrane-spanning helices, with the N and C termini being in the cytosol. This topology matches predictions from the latest prediction programs and the topology of the similar, recently crystallized membrane protein BtuC.