The DNA binding activity of Translin is mediated by a basic region in the ring‐shaped structure conserved in evolution

Open Access

29 January 1999

journal article
research article
Published by Wiley in FEBS Letters

Vol. 443 (3) , 363-366
https://doi.org/10.1016/s0014-5793(99)00010-1

Abstract

DNA binding proteins, for the most part, function as dimers or tetramers which recognize their target sequences. Here we show that Translin, a novel single‐stranded DNA end binding protein, forms a ring‐shaped structure conserved throughout evolution and that this structure is responsible for its DNA binding activity. Point mutations at Leu¹⁸⁴ and Leu¹⁹¹ in the leucine zipper motif of human Translin resulted in loss of the multimeric structure and abrogation of DNA binding. Point mutations at R⁸⁶, H⁸⁸, H⁹⁰ to T⁸⁶, N⁸⁸, N⁹⁰ in one of the basic regions, however, completely inhibited the DNA binding activity without affecting the multimeric structure. These results support the view that the DNA binding domain of Translin is formed in the ring‐shaped structure in combination with its basic region (amino acids 86–97) polypeptides.

Keywords

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