INVITRO CYTOTOXICITY OF SERUM AND PERIPHERAL-BLOOD LEUKOCYTES FOR EQUINE HERPESVIRUS TYPE 1-INFECTED TARGET-CELLS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (1) , 117-121
Abstract
The immune response in horses following experimental infection with equine herpesvirus type 1 (EHV-1) was assessed by measuring cytotoxicity for EHV-1-infected target cells. A technique was developed, using [125I]5-iodo-2''-deoxyuridine ([125I]IUDR)-labeled equine fetal kidney cells infected with EHV-1 as the target cells. Peripheral blood leukocytes from a recovered horse were capable of lysing target cells, as measured by the loss of radioactive label. Following the experimental infection of specific-pathogen-free ponies with EHV-1, cytotoxicity was obtained with fresh autologous serum, peripheral blood leukocytes in autologous serum and washed peripheral blood leukocytes. Cytotoxicity of the serum and peripheral blood leukocytes was detected as early as 1 day after infection. Cytotoxic antibodies or cells could play an important part in restricting virus spread after infection of the horse with EHV-1.

This publication has 1 reference indexed in Scilit: