A temperate bacteriophage of Clostridium perfringens

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Abstract
A temperate bacteriophage was isolated from a lysogenic strain of Clostridium perfringens. This lysogenic strain was inducible by ultraviolet light. Plaques produced on solid medium were small with turbid centers. Electron microscopy revealed that the phage has a polyhedral head, 80 × 70 mμ, and a striated tail, 200 mμ long.One-step growth experiments revealed latent and release periods of 45 minutes each. Phage yields were variable but, in general, quite low. The adsorption rate and temperature and pH stability of the phage were determined.The diphenylamine reaction and acridine orange staining suggested that the phage nucleic acid is deoxyribonucleic acid.

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