In Vitro Detection of Cells Infected with Lactic Dehydrogenase Virus (LDV) by Fluorescein-Labeled Antibody to LDV

Abstract
The percentage of cells infected with lactic dehydrogenase virus (LDV) in primary mouse embryo cells was studied by immunofluorescent and infectious center assays. Data from both techniques indicated that only a small percentage of cells in the monolayers was infected and that each infected cell yielded at least 4,000 ID50 of virus. The finding of immunofluorescent-positive cells in primary cultures provides an additional method for detecting LDV and eliminates the need to do all assays in vivo.

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