Directed Evolution of Enzymes for Biocatalytic Applications
- 1 January 2001
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis and Biotransformation
- Vol. 19 (2) , 85-97
- https://doi.org/10.3109/10242420109003638
Abstract
Directed molecular evolution is a rapidly growing field revolutionizing the development of biocatalysts with improved properties. This review describes methods to create mutant libraries and assays for rapid screening or selection of desired variants. Selected examples emphasizing the evolution of enzymes for applications in biocatalysis show that it is possible to alter substrate specificity, modulate enantioselectivity and increase enzyme performance under process conditions.Keywords
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