High Performance Liquid Affinity Chromatography (HPLAC) of Sterols with Tomatine Chemically Bonded to Microparticulate Silica
- 1 March 1986
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 9 (4) , 875-886
- https://doi.org/10.1080/01483918608076675
Abstract
A selective HPLAC stationary phase for the separation of sterols was achieved by the chemical bonding of tomatine to microporous silica. Columns packed with tomative slilica have given promising results indicating their usefulness as well for selective on-column enrichment as for HPLC separations. Good separation was achieved with a mixture of five sterols known as oxidation products of cholesterol.Keywords
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