Lymphoid cell surface receptor for Moloney leukemia virus envelope glycoprotein gp71. II. Isolation of the receptor.

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Abstract
The lymphoid cell surface receptor for Moloney leukemia virus envelope glycoprotein gp71 was isolated by using anti-gp71 antibodies to immunoprecipitate the receptor-gp71 complex from detergent extract of radiolabeled murine thymus cells. Upon sodium dodecyl sulfate gel electrophoresis, a single molecule with an apparent m.w. of 190,000 was identified as the putative gp71-receptor. Analysis in nonreducing conditions indicated that the receptor may be composed of at least 2 subunits of 190,000 daltons.