High-speed Liquid Chromatographic Determination of Glycyrrhizin in Liquorice Roots and Extracts

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Abstract
A method for the determination of glycyrrhizin in liquorice roots and its preparation was devised by the use of high-speed liquid chromatography. Separation can be achieved within 12 min employing a 1 m column packed with Permaphase AAX pellicular anion exchanger, using gradient from H2O to 0.05M NaClO4 solution as an eluant in a Du Pont LC 830 liquid chromatography. Accuracy in this method was found to be less than .+-. 1.5%, with the relation Y = 0.856X-0.538, and a relative coefficient of 0.999.

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