THE USE OF FLUORESCENT PROBES IN IMMUNOCHEMISTRY*
- 1 August 1990
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 52 (2) , 431-438
- https://doi.org/10.1111/j.1751-1097.1990.tb04200.x
Abstract
—: The limitations and advantages of particular dyes for labelling proteins and other biological materials are discussed. Methods available for conjugating dyes to proteins are outlined. Following a discussion of double labelling methods the use of photoactivatable fluorochromes and time resolved fluorescence methodologies are outlined. The reasons for the photoinstability of some fluorochromes are discussed and methods for overcoming the problem are described.This publication has 16 references indexed in Scilit:
- Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedyPublished by Elsevier ,2002
- A simple specific labelling for oligonucleotides by bathophenanthroline-RuII complexes as nonradioactive label moleculesTetrahedron Letters, 1989
- Photoactivable fluorophores. 2. Synthesis and photoactivation of functionalized 3-aroyl-2-(2-furyl)-chromones.Tetrahedron Letters, 1988
- Bathophenanthroline‐ruthenium(II) complexes as non‐radioactive labels for oligonucleotides which can be measured by time‐resolved fluorescence techniquesHelvetica Chimica Acta, 1988
- Fluorescence quenching of fluorescein by R-phycoerythrinJournal of Immunological Methods, 1988
- The effects of enzymes on the photobleaching of fluorescein and fluorescein isothiocyanate conjugatesJournal of Photochemistry and Photobiology B: Biology, 1988
- Photoactivable fluorophores. 1. Synthesis and photoactivation ofo-nitrobenzyl-quenched fluorescent carbamates.Tetrahedron Letters, 1988
- Energy Transfer Luminescence of Europium(III) and Terbium(III) Cryptates of Macrobicyclic Polypyridine LigandsAngewandte Chemie International Edition in English, 1987
- "Second-sphere" photochemistry and photophysics of coordination compoundsChemical Reviews, 1986
- Phycofluor probesTrends in Biochemical Sciences, 1984