RELATIONSHIP OF SULFAMETHAZINE IN SWINE DIETS AND RESULTANT TISSUE CONCENTRATIONS, USING TISHLER AND GAS-LIQUID-CHROMATOGRAPHIC METHODS

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 42  (2) , 339-343
Abstract
Sulfamethazine (110 mg/kg feed) was fed to 4 groups of pigs for 10 days. Each group was then fed withdrawal rations containing 0, 1.1, 11.0 or 110.0 mg/kg feed for 10 days before slaughter. A 5th group was fed a sulfamethazine-free diet only. Fat, kidney, liver and muscle from each pig were analyzed by several laboratories, using the Tishler Bratton-Marshall (BM) and GLC methods. Mean background (BG) amounts of sulfamethazine, as measured in control tissues, were negligible using GLC. BM BG values (mean .+-. SD), expressed as milligrams sulfamethazine/kg edible animal tissues (ppm) were: fat 0.019 .+-. 0.003, kidney 0.048 .+-. 0.012, liver 0.053 .+-. 0.024 and muscle 0.018 .+-. 0.017. Regression of tissue sulfamethazine on feed sulfamethazine, as measured by GLC and BM adjusted for BM-BG values yielded linear plots. Values obtained by GLC and corrected BM were highly correlated but variability was higher using GLC. The linear model predicted that dietary sulfamethazine required to produce 0.1 ppm in liver as measured by BM, BM-BG and GLC were 1.6, 2.8 and 3.4 ppm, respectively. Concentrations necessary to produce 0.1 ppm in muscle were 5.3, 6.2 and 5.5 ppm. TLC method of screening swine plasma was a good predictor of tissue sulfamethazine values.