The activation of nuclear phosphoinositide 3‐kinase C2β in all‐trans‐retinoic acid‐differentiated HL‐60 cells
- 16 September 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 529 (2-3) , 268-274
- https://doi.org/10.1016/s0014-5793(02)03357-4
Abstract
The activity of nuclear phosphoinositide 3-kinase C2β (PI3K-C2β) was investigated in HL-60 cells induced to differentiate along granulocytic or monocytic lineages. A significant increase in the activity of immunoprecipitated PI3K-C2β was observed in the nuclei and nuclear envelopes isolated from all-trans-retinoic acid (ATRA)-differentiated cells which was inhibited by the presence of PI3K inhibitor LY 294002. High-performance liquid chromatography analysis of inositol lipids showed an increased incorporation of radiolabelled phosphate in both PtdIns(3)P and PtdIns(3,4,5)P3 with no changes in the levels of PtdIns(4)P, PtdIns(3,4)P2 and PtdIns(4,5)P2. Western blot analysis of the PI3K-C2β immunoprecipitates with anti-P-Tyr antibody revealed a significant increase in the level of the immunoreactive band corresponding to PI3K-C2β in the nuclei and nuclear envelopes isolated from ATRA-differentiated cellsKeywords
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