UPTAKE OF URIC-ACID BY SEPARATED RENAL TUBULES OF RABBIT .1. CHARACTERISTICS OF TRANSPORT

  • 1 January 1977
    • journal article
    • research article
    • Vol. 201  (1) , 218-225
Abstract
A rapid filtration procedure was used to determine rates of uric acid uptake by a preparation of separated renal cortical tubules of the rabbit. The rate of uric acid uptake was temperature dependent and showed saturation kinetics with a Km of 3.2 mM. The uptake was 50% lower when measured under N2 as compared with uptake under O2. The uptake rate increased with increasing Na concentration and decreased with increasing K concentration. Uptake was stimulated by citrate, succinate and pyruvate, and inhibited by .alpha.-ketoglutarate. Parathyroid hormone and 10-4 M cyclic AMP increased the uric acid uptake rate when preincubated with the tubules for 130 min before the addition of uric acid. Uric acid uptake in this preparation may occur by some form of carrier-mediated active transport.

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