Determination of maintenance coefficients ofSaccharomyces cerevisiae cultures with cell recycle by cross-flow membrane filtration

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Abstract
The fermentation of glucose by a strain of Saccharomyces cerevisiae was studied in a continuous single‐stage process with recycle of the cells via cross‐flow micro‐filtration membranes. Operating conditions were selected such that the culture was not carbon limited and inhibition by ethanol and cell death were minimized. Steady states were obtained for various biomass bleeding rates, i.e., various specific growth rates. From the experimental data, the stoichiometry of the simultaneous reactions, cell growth, ethanol production and maintenance were established using mass and degree of reduction balance relative to substrates (carbon source and oxygen) and products (biomass, ethanol, carbon dioxide etc.), and the growth parameters, yields, and maintenance cofficients were determined. It was shown that the oxygen consumption was not linked to the kinetics of the fermentation. The calculated growth constants were discussed and compared to the currently reported values.