Evaluation of the Chromatographic Behaviour of Fluoxetine and Norfluoxetine Using Different Cyclodextrins as Mobile Phase Additives and Fluorimetric Detection

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Abstract
The principal goal of this work was to investigate the liquid chromatographic retention behaviour of fluoxetine and norfluoxetine using HPLC with respect to mobile phase composition, pH, flow rate and the amount of the native β-cyclodextrin (β-CD) or the β-hydroxypropyl-cyclodextrin (HP-β-CD), added to the mobile phase. Further, it was of interest to evaluate the effectiveness of β-CD and HP-β-CD to enhance fluorescence detection of these compounds. Another purpose of this study, was to calculate the formation constants of the inclusion complexes (Kf) of fluoxetine and norfluoxetine within the HP-β-CD, in different mobile phase compositions. Based on these findings, the log Kf values of these two compounds referred to pure aqueous mobile phase (log Kfw), can be determined by extrapolation.

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