Alanyl-tRNA synthetase gene of the extreme acidophilic chernolithoautotrophic Thiobacillus ferrooxidans is highly homologous to alaS genes from all living kingdoms but cannot be transcribed from its promoter in Escherichia coli
Open Access
- 1 July 1997
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 143 (7) , 2179-2187
- https://doi.org/10.1099/00221287-143-7-2179
Abstract
Summary: The alaS gene of Thiobacillus ferrooxidans has been cloned and sequenced and its expression in Escherichia coli and T. ferrooxidans analysed. The same genomic organization to that in E. coli (recA-recX-alaS) has been found in T. ferrooxidans. The recA and alaS genes cannot be transcribed from their own promoters in E. coli. In addition to the well-known homology at the protein level between AlaS proteins from various organisms, a strong homology was found between all the known alaS genes from bacteria, archaea and eucarya. Two regions, one of which corresponds to the catalytic core, are particularly well-conserved at the nucleotide sequence level, a possible indication of strong constraints during evolution on these parts of the genes.Keywords
This publication has 41 references indexed in Scilit:
- The F1genes of the F1F0ATP synthase from the acidophilic bacteriumThiobacillus ferrooxidanscomplementEscherichia coliF1uncmutantsFEMS Microbiology Letters, 1994
- Functional Dissection of a Predicted Class-Defining Motif in a Class II tRNA Synthetase of Unknown StructureBiochemistry, 1994
- A putative regulatory gene downstream ofrecA is conserved in Gram-negative and Gram-positive bacteriaNucleic Acids Research, 1994
- Chapter 9 Mineral theories of the origin of life and an iron sulfide exampleDiscover Life, 1992
- Primary structure of alanyl-tRNA synthetase and the regulation of its mRNA levels in Bombyx mori.Published by Elsevier ,1990
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifsNature, 1990
- Nitrate reductase of Escherichia coli: Completion of the nucleotide sequence of the nar operon and reassessment of the role of the α and β subunits in iron binding and electron transferMolecular Genetics and Genomics, 1989
- One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.Proceedings of the National Academy of Sciences, 1989
- A comprehensive set of sequence analysis programs for the VAXNucleic Acids Research, 1984