RNA polymerase rifampicin resistance mutations in Escherichia coli: Sequence changes and dominance
- 1 May 1983
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 190 (2) , 344-348
- https://doi.org/10.1007/bf00330662
Abstract
Summary Five recombinant plasmids, pBK2646, pBK611, pRC3, pRC4 and pRC5, carrying rpoB rifampicin-resistant RNA-polymerase genes were obtained. The sequence analysis of these plasmids revealed certain structural changes in the rpoB gene which specify corresponding alterations in the β-subunit of RNA polymerase. Some functional properties of the corresponding mutant strains and their RNA polymerases have been investigated.Keywords
This publication has 21 references indexed in Scilit:
- Primary structure of Escherichia coli RNA polymerase nucleotide substitution in the β subunit gene of the rifampicin resistant rpoB255 mutantMolecular Genetics and Genomics, 1981
- Subunits of E. coli RNA polymerase forming 3′-OH binding site at initial stages of transcription processFEBS Letters, 1980
- Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12Molecular Genetics and Genomics, 1980
- The two effects of rifampicin on the RNA polymerase reactionBiochemical and Biophysical Research Communications, 1977
- The Modification of DNA‐Dependent RNA Polymerase from Escherichia coli by an Alkylating Derivative of Rifamycin SVEuropean Journal of Biochemistry, 1975
- A temperature sensitive mutation of the β′‐subunit of DNA‐dependent RNA polymerase from E. coli T16FEBS Letters, 1974
- RNA polymerase mutants of Escherichia coliMolecular Genetics and Genomics, 1973
- Propriétés de dominance de quelques mutations conférant la résistance à la rifampicine chez E. coli K12Biochimie, 1971
- Reconstitution of bacterial DNA‐dependent RNA‐polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcriptionFEBS Letters, 1970
- On the process of cellular division in Escherichia coliJournal of Molecular Biology, 1968