Effects ofin vivo administration of detergents on the hepatic microsomal cytochrome P-450 system in rat

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Abstract
The influence of in vivo administration of detergents on the hepatic microsomal cytochrome P‐450 system was studied in rat. Male Wistar rats were administered detergents, Emulgen 913 (50 mg or 100 mg kg−1 of body weight (B.W.)), or sodium dodecylsulfate (SDS, 25 mg or 50 mg kg−1 of B.W.) intraperitoneally once a day for 3 days. Cytochrome P‐450 content in liver microsomes was significantly decreased to 85% and 73% of control by the administration of 50 mg or 100 mg Emulgen, respectively, but the microsomal protein concentration was not changed by these administrations. The content of cytochrome P‐450 was also reduced to 76% and 70% of control by the administration of 25 mg or 50 mg SDS/kg of B.W., respectively. The total hydroxylation activity (the sum of ω‐ and (ω ‐ 1)‐hydroxylase activity) of laurate almost paralleled the decrease in cytochrome P‐450 in detergent‐treated rats. However, the ω/ω‐1‐hydroxylation ratio was not changed. These results suggest that the administration of these detergents lowered the level of cytochrome P‐450 species catalyzing ω‐ and (ω ‐ 1)‐hydroxylation of laurate to a similar extent. On the other hand, aminopyrine N‐ and p‐nitroanisole O‐demethylation activities in Emulgen 913‐treated rats was decreased while those in SDS‐treated rats did not change, though the content of cytochrome P‐450 was decreased by both administrations. Thus, it was demonstrated that the livers of rats responded to exogenous detergents in different manners.

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