Luciferase cDNA from Japanese firefly, Luciola cruciata: Cloning, structure and expression in Escherichia coli
- 1 April 1989
- journal article
- conference paper
- Published by Wiley in Journal of Bioluminescence and Chemiluminescence
- Vol. 3 (2) , 75-78
- https://doi.org/10.1002/bio.1170030208
Abstract
We have cloned the cDNA for luciferase from lantern poly(A)+ RNA of a Japanese firefly, Luciola cruciata (Genji botaru in Japanese). This cDNA directed the synthesis of enzymatically active luciferase under the control of the lac promoter in Escherichia coli. The amino acid sequence predicted from the cDNA sequence shows that Genji firefly luciferase consists of 548 amino acids and has a molecular weight of 60,024. Considerable sequence homology was found upon the comparison of the Genji and North American firefly luciferases.Keywords
This publication has 6 references indexed in Scilit:
- Synthesis of enzymatically active firefly luciferase in yeast.Agricultural and Biological Chemistry, 1988
- Firefly luciferase gene: structure and expression in mammalian cells.Molecular and Cellular Biology, 1987
- [1] Cloning firefly luciferasePublished by Elsevier ,1986
- Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.Proceedings of the National Academy of Sciences, 1985
- [1] Purification and properties of firefly luciferasePublished by Elsevier ,1978
- Luciferin and luciopterin isolated from the japanese firefly,Tetrahedron Letters, 1968