Identification and Isolation of Lewis Blood Group Antigens from Human Saliva Using Monoclonal Antibodies
- 1 January 1984
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 3 (1) , 1-10
- https://doi.org/10.1089/hyb.1984.3.1
Abstract
Solid-phase radioimmunoassay, polyacrylamide gel electrophoresis and thin-layer chromatography were used to compare, identify, and characterize the Lewis antigens from human salivas, using monoclonal antibodies directed to the Lea and Leb determinants. Sialylated Lea glycolipid was detected in saliva from individuals with Le(a+b+) and Le(a+b-) phenotypes. Immunoaffinity chromatography of the saliva from individuals with different phenotypes revealed a glycoprotein of molecular weight greater than 200 kD bearing the Lewis antigenic determinants.Keywords
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