Abstract
A washing and analytical procedure for the analysis of the zinc and copper concentrations of hair samples is described. The treatment of samples with a nonionic detergent + EDTA wash removed the most adsorbed zinc and copper from hair and was the washing procedure chosen for routine analyses. Beauty treatments had a variable effect on hair zinc and copper concentrations; the most drastic procedures were cold waving and bleaching; conditioner, hair spray, tint, and a permanent dye had a less drastic or no effect. Increasing distance of the hair from the scalp was associated with a regular increase in copper concentration and also increases in zinc concentration for most subjects.

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