The Xenopus laevis mitochondrial protein mtDBP-C cooperatively folds the DNA in vitro.
Open Access
- 1 December 1988
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 7 (12) , 3873-3879
- https://doi.org/10.1002/j.1460-2075.1988.tb03273.x
Abstract
The binding of the Xenopus laevis mitochondrial protein mtDBP‐C to DNA was studied by equilibrium density banding, agarose gel electrophoresis and electron microscopy. The results obtained show that the mtDBP‐C binds cooperatively to DNA irrespective of whether the DNA is supercoiled, relaxed or linear and it induces the formation of superhelical turns locally leading to the formation of a highly folded structure. It appears that this protein could be involved in the compaction of DNA in the mitochondrial nucleoid.This publication has 23 references indexed in Scilit:
- Structure of the nucleosome core particle at 7 Å resolutionNature, 1984
- Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli.Proceedings of the National Academy of Sciences, 1984
- Mitochondrial DNA-binding proteins that bind preferentially to supercoiled molecules containing the D-loop region of Xenopus laevis mtDNABiochemical and Biophysical Research Communications, 1983
- Changes in D-loop frequency and superhelicity among the mitochondrial DNA molecules in relation to organelle biogenesis in oocytes ofExperimental Cell Research, 1983
- A High-resolution electron microscopy study of nucleosomes from simian virus 40 chromatinJournal of Molecular Biology, 1981
- Single-strand deoxyribonucleic acid binding protein from rat liver changes the helical structure of deoxyribonucleic acidBiochemistry, 1981
- A DNA binding protein from Xenopus laevis oocyte mitochondriaChromosoma, 1981
- Characterization of a histone-like protein extracted from yeast mitochondria.Proceedings of the National Academy of Sciences, 1979
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A new preparation method for dark-field electron microscopy of biomacromoleculesJournal of Ultrastructure Research, 1971