The Laboratory Colonization of Culicoides variipennis (Coq.)1

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Abstract
This species of biting midge was reared through several successive generations in the laboratory. The main colony is now on its 6th laboratory generation and is producing adult flies at the rate of about 100 per day. Nourishment consists of water and sugar for the males and, in addition, blood for the females. A rabbit is used in providing blood meals, as the adult females readily engorge from the inner surface of the ears. The larval medium consists of fresh cow manure mixed with soil and water. All stages of the colonies are kept in a constant temperature room at 75[degree] F with a relative humidity of 50%. The life cycle in the laboratory is about 30 days in duration, the approximate breakdown being as follows: egg, 2; larva, 23; pupa, 2; and pre-oviposition, 3.

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