LIPOPOLYSACCHARIDES OF THE HEDDLESTON SEROTYPES OF PASTEURELLA-MULTOCIDA

  • 1 January 1984
    • journal article
    • research article
    • Vol. 45  (4) , 759-763
Abstract
Lipopolysaccharides (LPS) were extracted from 13 of the 16 Heddleston serotypes of P. multocida by phenol-CHCl3-petroleum either (PCP). Serotypes 3, 9 and 13 were extracted only by phenol-H2O (PW). After extraction of LPS of serotype 9 by PW, an additional LPS was isolated by PCP. All LPS contained glucose, 2-keto-3-deoxyoctonate and heptose. Two isomers of heptose, D-glycero-D-mannoheptose and L-glycero-D-mannoheptose, were found in serotypes 2 and 5. Antisera made against purified LPS of serotypes 2 and 5 reacted with heat-stable antigens and LPS from serotypes 2 and 5 in the gel-diffusion precipitin test. Antisera against serotype 2 LPS protected turkeys against challenge with capsulated serotype 5, indicating that a structural relationship exists between LPS of strains that cause hemorrhagic septicemia and fowl cholera. Rhamnose was a component of serotype 9 LPS; galactose was found in all LPS, except for serotype 11. The LPS of serotype 13 contained an isomer of heptose that has not been identified. The LPS had buoyant densities in CsCl of 1.40 .+-. 0.0148 g/ml; all hemagglutinated chicken and turkey, but not sheep or horse, red blood cells.