In vitro Cultivation of Dipetalonema viteae Third-Stage Larvae: Evaluation of Culture Media, Serum, and Other Supplements

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Abstract
3rd-Stage lavae of D. viteae obtained from the tick vector developed to the 4th stage in several cell-free culture systems. Survival and development of larvae in a number of commercially available cell culture media, supplemented with serum and other defined and undefined components, were compared. All cultures were gassed with 5% CO2 in N. Best survival, growth and development were obtained in stationary cultures containing 1:1 (v/v) mixtures of NCTC 135, either RPMI 1640 or Iscove''s Modified Dulbecco''s Medium, and a supplement of 20% non-heat-inactivated fetal bovine serum. The importance of the medium composition and physical environment of the culture system for the survival, growth and development of D. viteae was demonstated.