Further Studies of the Germ-Tube Test forCandida albicansIdentification

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Abstract
An evaluation of the optimal incubation time for the germ-tube test used in the rapid identification of Candida albicans revealed that a 2-hr. incubation will identify 78% of the strains, whereas a 3-hr. incubation will identify 94% of the strains tested. Extending the incubation to 4 hr. increases the strains identified by only 1%. A study of the comparative efficiency of human and sheep sera in producing germ tubes revealed that both were equally effective. However, the cost of commercially obtained sheep serum is about one third that of human serum; thus, it is more economical to use sheep serum.

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