DNA. swivel enzyme activity in a nuclear membrane fraction
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (1) , 223-228
- https://doi.org/10.1093/nar/4.1.223
Abstract
DNA swivel (nicking-rejoining) enzyme activity has been studied in various cell fractions of a human lymphoid cell line. Swivel activity is found only in chromatin and in a nuclear membrane fraction containing DNA and possessing endogenous DNA synthesizing activity. Twenty percent of the total swivel activity and less than one percent of the total DNA are in the membrane fraction. The swivel enzyme is more tightly bound to the membrane fraction than to the chromatin fraction. These observations suggest that the swivel enzyme may be a replication factor, specifically bound to replicating DNA in the membrane fraction.Keywords
This publication has 9 references indexed in Scilit:
- Purification and demonstration of the enzymatic character of the nicking-closing protein from mouse L cellsBiochemical and Biophysical Research Communications, 1976
- Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.Proceedings of the National Academy of Sciences, 1975
- New DNA polymerase activity associated with nuclear membranes from a human lymphoid cell lineBiochemical and Biophysical Research Communications, 1975
- Characterization of purified DNA-relaxing enzyme from human tissue culture cells.Proceedings of the National Academy of Sciences, 1975
- Isolation and Partial Characterisation of the Relaxation Protein from Nuclei of Cultured Mouse and Human CellsEuropean Journal of Biochemistry, 1975
- ι Protein from Drosophila melanogasterBiochemistry, 1974
- Stepwise Relaxation of Supercoiled SV40 DNACold Spring Harbor Symposia on Quantitative Biology, 1974
- An Activity from Mammalian Cells That Untwists Superhelical DNA—A Possible Swivel For DNA ReplicationProceedings of the National Academy of Sciences, 1972
- Interaction between DNA and an Escherichia coli protein ωJournal of Molecular Biology, 1971